Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K
نویسندگان
چکیده
منابع مشابه
A protease stable in organic solvents from solvent tolerant strain of Pseudomonas aeruginosa.
A solvent tolerant strain of Pseudomonas aeruginosa (PseA) was isolated from soil samples by cyclohexane enrichment in medium. The strain was able to sustain and grow in a wide range of organic solvents. The adaptation of P. aeruginosa cell towards solvents was seen at membrane level in transmission electron micrographs. It also secreted a novel protease, which exhibited remarkable solvent stab...
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BACKGROUND Many researchers have reported on the optimization of protease production; nevertheless, only a few have reported on the optimization of the production of organic solvent-tolerant proteases. Ironically, none has reported on thermostable organic solvent-tolerant protease to date. The aim of this study was to isolate the thermostable organic solvent-tolerant protease and identify the c...
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A highly sensitive and specific radioimmunoassay has been developed for Pseudomonas aeruginosa alkaline protease. Production of alkaline protease was found to be strain variable and medium dependent.
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Seeding is a versatile method for optimizing crystal growth. Coupling this technique with capillary counter diffusion crystallization enhances the size and diffraction quality of the crystals. In this article, crystals for organic solvent-tolerant recombinant elastase strain K were successfully produced through microseeding with capillary counter-diffusion crystallization. This technique improv...
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An organic solvent-tolerant S5 lipase was purified by affinity chromatography and anion exchange chromatography. The molecular mass of the lipase was estimated to be 60 kDa with 387 purification fold. The optimal temperature and pH were 45 degrees C and 9.0, respectively. The purified lipase was stable at 45 degrees C and pH 6-9. It exhibited the highest stability in the presence of various org...
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ژورنال
عنوان ژورنال: Bioresource Technology
سال: 2005
ISSN: 0960-8524
DOI: 10.1016/j.biortech.2004.06.012